The claim is that DeSantis is being a hypocrite when he backs trucker protests that intend to block roads, because he backed and signed an anti-rioting law that stiffened penalties for protesters who blocked roads and even gave some legal protection to drivers who ran them over. The Federal courts, however struck down the law as being unconstitutional.
I don’t think that it makes you a hypocrite to play by the rules that have been put in place, even if you disagree with those rules. DeSantis tried to make road blocking protests illegal and was overruled by the court. Since those are now the rules, it is not hypocritical to play by those rules.